rabbit vegfa polyclonal ab  (EnoGene Inc)

Journal: Archives of Medical Science : AMS

Article Title: Therapeutic potential of mesenchymal stem cells in cisplatin-induced acute kidney injury via ASK-1/TXNIP pathway modulation

doi: 10.5114/aoms/193707

Figure Lengend Snippet: Immunohistochemical staining of VEGF of renal cortex sections at magnification ×400, bar 25 µm. A – Immunohistochemical-stained kidney sections of control group showing strong reaction of VEGF protein expression in the renal cortex. B – Immunohistochemical-stained kidney sections of AKI group showing significant reduction in the expression of VEGF in renal medulla. C – Immunohistochemical-stained kidney sections for VEGF protein expression of FUR group showing mild increase in the expression of VEGF in renal cortex. D – Immunohistochemical-stained kidney sections for VEGF protein expression of MSCs group showing marked increase in the expression of VEGF in renal medulla E – The angiogenic effects of MSC in kidney tissue, expressed as area %. Data were presented as means ± SEM. Each column represented the means ± SEM. The mean variations between the groups using Turkey’s significant difference test. a p < 0.01 vs. control, b p < 0.01 vs. cisplatin induced acute kidney injury cells, c p < 0.01 vs. furosemide treated cells

Article Snippet: Hematoxylin and eosin (H&E) stain was applied to 5 μm sections of paraffin-embedded renal tissues for histological investigation [ ], using anti-VEGF rabbit polyclonal Ab (Boster Biological Technology, Pleasanton, CA, USA, Cat.# PA1080).

Techniques: Immunohistochemical staining, Staining, Control, Expressing

Journal: iScience

Article Title: Revisiting the effects of exercise on cerebral neurovascular functions in rats using multimodal assessment techniques

doi: 10.1016/j.isci.2023.106354

Figure Lengend Snippet: Key resources table

Article Snippet: Rabbit polyclonal anti-VEGFA , Elabscience , Cat# E-AB-64001; RRID:N/A.

Techniques: Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Western Blot, Software

Journal: PLoS ONE

Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

doi: 10.1371/journal.pone.0148774

Figure Lengend Snippet: Genes regulated by ZEB1.

Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

Techniques: Control

Journal: PLoS ONE

Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

doi: 10.1371/journal.pone.0148774

Figure Lengend Snippet: (A) MDA-MB-231 cells were transiently transfected with human ZEB1 expression plasmid or empty vector control. At the indicated time points, expression of ZEB1, VEGFA and VEGFC was verified by Western blotting. Actin was used as a loading control. (B) Production of VEGFA and VEGFC protein were verified by ELISA at the indicated time points following ZEB1 overexpression. * P < 0.05, ** P < 0.01 vs respective control in one-way ANOVA followed by Tukey’s Honestly Significant Difference test. (C) HUVECs cultured in the presence or absence of VEGFA (20 and 40 ng/mL) or anti-VEGFA neutralized Ab (1 μg/mL) along with ZEB1/231-derived conditioned medium were subjected to a tube formation assay and photographed. (D) Quantification of tube formation was expressed as length of capillary tubes formed per mm 2 . * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

Techniques: Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Enzyme-linked Immunosorbent Assay, Over Expression, Cell Culture, Derivative Assay, Tube Formation Assay

Journal: PLoS ONE

Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

doi: 10.1371/journal.pone.0148774

Figure Lengend Snippet: Positive correlation between ZEB1 and VEGFA expression in breast cancer.

Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

Techniques: Expressing

Journal: PLoS ONE

Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

doi: 10.1371/journal.pone.0148774

Figure Lengend Snippet: The non-negative percentage analysis for VEGFA (A) and CD31 (B) indicates a positive correlation with ZEB1 expression in breast cancer tumors from 228 subjects. (C) Representative images of immunohistochemical staining of ZEB1, VEGFA, and CD31 in tumors from 4 cases are shown. Scale bars, 25 μm.

Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: PLoS ONE

Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

doi: 10.1371/journal.pone.0148774

Figure Lengend Snippet: MDA-MB-231 cells were transiently transfected with the human ZEB1 expression plasmid or empty vector control, followed by treatment with PI-103 (10 μM) or SB203580 (10 μM). At the indicated time points, upregulation of VEGFA expression were verified by qPCR (A and B), Western blotting (C and D) and ELISA (E and F) in ZEB1-expressing versus control cells. GAPDH and actin were used to normalize VEGFA levels. * P < 0.05, ** P < 0.01 vs respective control in Student’s t -test.

Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

Techniques: Transfection, Expressing, Plasmid Preparation, Control, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: PLoS ONE

Article Title: ZEB1 Upregulates VEGF Expression and Stimulates Angiogenesis in Breast Cancer

doi: 10.1371/journal.pone.0148774

Figure Lengend Snippet: (A) Sequential deletion and mutation of SP1 elements on the human VEGFA promoter were fused to the luciferase reporter. (B) MDA-MB-231 cells were co-transfected with the ZEB1 expression plasmid (1 μg/well) and different wild-type VEGFA promoter luciferase reporter constructs (1 μg/well). Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values were normalized to Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (C) MDA-MB-231 cells were co-transfected with ZEB1 expression plasmid and wild-type or mutant VEGFA promoter luciferase reporters. Extract luciferase activities were determined 48 h after transfection using a Betascope analyzer. Luciferase values are normalized with Renilla activities. * P < 0.05 vs respective control in Student’s t -test. (D) The association of SP1 with the proximal human VEGFA promoter was analyzed by ChIP assay, using polyclonal Ab against SP1 or unrelated IgG Ab. The amplified sequence of the VEGFA promoter fragment containing SP1 elements is shown. Input DNA amounts were confirmed by equal loading of chromatin. (E) Overexpression of ZEB1 significantly enhanced the recruitment of SP1 to the endogenous VEGFA promoter as confirmed by a quantitative ChIP assay. * P < 0.05 vs respective control in Student’s t -test.

Article Snippet: The following antibodies were used: goat polyclonal Ab against ZEB1 (ab81972; Abcam) at dilution of 1:1000, rabbit polyclonal Ab against VEGFA (19003-1-AP; Proteintech) at dilution of 1:1000, rabbit polyclonal Ab against VEGFC (22601-1-AP; Proteintech) at dilution of 1:500, and mouse monoclonal Ab against actin (A-4700; Sigma) at dilution of 1:1000.

Techniques: Mutagenesis, Luciferase, Transfection, Expressing, Plasmid Preparation, Construct, Control, Amplification, Sequencing, Over Expression